Establishing a versatile toolkit of flux enhanced strains and cell extracts for pathway prototyping
Metabolic Engineering Xiunan Yi, Blake J Rasor, Nathalie Boadi, Katherine Louie, Trent R Northen, Ashty S Karim, Michael C Jewett, Hal S Alper
2023
At-home, cell-free synthetic biology education modules for transcriptional regulation and environmental water quality monitoring
ACS synthetic biology Jaeyoung K Jung, Blake J Rasor, Grant A Rybnicky, Adam D Silverman, Janet Standeven, Robert Kuhn, Teresa Granito, Holly M Ekas, Brenda M Wang, Ashty S Karim, Julius B Lucks, Michael C Jewett
A rapid cell-free expression and screening platform for antibody discovery
Nature communications Andrew C Hunt, Bastian Vögeli, Ahmed O Hassan, Laura Guerrero, Weston Kightlinger, Danielle J Yoesep, Antje Krüger, Madison DeWinter, Michael S Diamond, Ashty S Karim, Michael C Jewett
A rapid cell-free expression and screening platform for antibody discovery Read More »
A Cell-Free Gene Expression Platform for Discovering and Characterizing Stop Codon Suppressing tRNAs
ACS Chemical Biology Kosuke Seki, Joey L Galindo, Ashty S Karim, Michael C Jewett
Point-of-care peptide hormone production enabled by cell-free protein synthesis
ACS Synthetic Biology Madison A DeWinter, Ariel Helms Thames, Laura Guerrero, Weston Kightlinger, Ashty S Karim, Michael C Jewett
Point-of-care peptide hormone production enabled by cell-free protein synthesis Read More »
A dynamic kinetic model captures cell-free metabolism for improved butanol production
Metabolic Engineering Jacob P Martin, Blake J Rasor, Jonathon DeBonis, Ashty S Karim, Michael C Jewett, Keith EJ Tyo, Linda J Broadbelt
A dynamic kinetic model captures cell-free metabolism for improved butanol production Read More »
Cell extracts from bacteria and yeast retain metabolic activity after extended storage and repeated thawing
ACS Synthetic Biology Blake J Rasor, Ashty S Karim, Hal S Alper, Michael C Jewett
Mechanistic insights into cell-free gene expression through an integrated-omics analysis of extract processing methods
ACS Synthetic Biology Blake J Rasor, Payal Chirania, Grant A Rybnicky, Richard J Giannone, Nancy L Engle, Timothy J Tschaplinski, Ashty S Karim, Robert L Hettich, Michael C Jewett