Semiautomated Production of Cell-Free Biosensors
ACS Synthetic Biology
Dylan M Brown, Daniel A Phillips, David C Garcia, Anibal Arce, Tyler Lucci, John P Davies Jr, Jacob T Mangini, Katherine A Rhea, Casey B Bernhards, John R Biondo, Steven M Blum, Stephanie D Cole, Jennifer A Lee, Marilyn S Lee, Nathan D McDonald, Brenda Wang, Dale L Perdue, Xavier S Bower, Walter Thavarajah, Ashty S Karim, Matthew W Lux, Michael C Jewett, Aleksandr E Miklos, Julius B Lucks
Cell-Free Expression of Soluble Leafhopper Proteins from BrochosomesNature Communications
ACS Synthetic Biology
Caleb G Lay, Gabriel R Burks, Zheng Li, Jeffrey E Barrick, Charles M Schroeder, Ashty S Karim, Michael C Jewett
Accelerated enzyme engineering by machine-learning guided cell-free expression
Nature Communications
Grant M Landwehr, Jonathan W Bogart, Carol Magalhaes, Eric G Hammarlund, Ashty S Karim, Michael C Jewett
Cell-free gene expression: methods and applications
ACS Synthetic Biology
Andrew C Hunt, Blake J Rasor, Kosuke Seki, Holly M Ekas, Katherine F Warfel, Ashty S Karim, Michael C Jewett
An automated cell-free workflow for transcription factor engineering
ACS Synthetic Biology
Holly M Ekas, Brenda Wang, Adam D Silverman, Julius B Lucks, Ashty S Karim, Michael C Jewett
Engineering a PbrR-based biosensor for cell-free detection of lead at the legal limit
ACS Synthetic Biology
Holly M Ekas, Brenda Wang, Adam D Silverman, Julius B Lucks, Ashty S Karim, Michael C Jewett
Deconstructing synthetic biology across scales: a conceptual approach for training synthetic biologists
Nature Communications
Ashty S Karim, Dylan M Brown, Chloé M Archuleta, Sharisse Grannan, Ludmilla Aristilde, Yogesh Goyal, Josh N Leonard, Niall M Mangan, Arthur Prindle, Gabriel J Rocklin, Keith J Tyo, Laurie Zoloth, Michael C Jewett, Susanna Calkins, Neha P Kamat, Danielle Tullman-Ercek, Julius B Lucks
A cell-free workflow for detecting and characterizing RiPP recognition element-precursor peptide interactions
bioRxiv
Derek A Wong, Maria D Cabezas, Martin Daniel-Ivad, Deepali V Prasanna, Regina Fernandez, Robert Nicol, Emily P Balskus, Ashty S Karim, Michael C Jewett
Ribosome Pool Engineering Increases Protein Biosynthesis Yields
ACS Central Science
Camila Kofman, Jessica A Willi, Ashty S Karim, Michael C Jewett
Establishing a versatile toolkit of flux enhanced strains and cell extracts for pathway prototyping
Metabolic Engineering
Xiunan Yi, Blake J Rasor, Nathalie Boadi, Katherine Louie, Trent R Northen, Ashty S Karim, Michael C Jewett, Hal S Alper
At-home, cell-free synthetic biology education modules for transcriptional regulation and environmental water quality monitoring
ACS synthetic biology
Jaeyoung K Jung, Blake J Rasor, Grant A Rybnicky, Adam D Silverman, Janet Standeven, Robert Kuhn, Teresa Granito, Holly M Ekas, Brenda M Wang, Ashty S Karim, Julius B Lucks, Michael C Jewett
A rapid cell-free expression and screening platform for antibody discovery
Nature communications
Andrew C Hunt, Bastian Vögeli, Ahmed O Hassan, Laura Guerrero, Weston Kightlinger, Danielle J Yoesep, Antje Krüger, Madison DeWinter, Michael S Diamond, Ashty S Karim, Michael C Jewett
A Cell-Free Gene Expression Platform for Discovering and Characterizing Stop Codon Suppressing tRNAs
ACS Chemical Biology
Kosuke Seki, Joey L Galindo, Ashty S Karim, Michael C Jewett
Point-of-care peptide hormone production enabled by cell-free protein synthesis
ACS Synthetic Biology
Madison A DeWinter, Ariel Helms Thames, Laura Guerrero, Weston Kightlinger, Ashty S Karim, Michael C Jewett
A dynamic kinetic model captures cell-free metabolism for improved butanol production
Metabolic Engineering
Jacob P Martin, Blake J Rasor, Jonathon DeBonis, Ashty S Karim, Michael C Jewett, Keith EJ Tyo, Linda J Broadbelt
Cell extracts from bacteria and yeast retain metabolic activity after extended storage and repeated thawing
ACS Synthetic Biology
Blake J Rasor, Ashty S Karim, Hal S Alper, Michael C Jewett
Mechanistic insights into cell-free gene expression through an integrated-omics analysis of extract processing methods
ACS Synthetic Biology
Blake J Rasor, Payal Chirania, Grant A Rybnicky, Richard J Giannone, Nancy L Engle, Timothy J Tschaplinski, Ashty S Karim, Robert L Hettich, Michael C Jewett